2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Isoform Specific Products:

DNA/RNA Synthesis Isoform Comparison
Cat. No. Product Name Effect Purity Chemical Structure
  • HY-N7225
    Yuanhuacine
    		99.79%
    Yuanhuacine (Gnidilatidin), a diterpene from Daphne genkwa, is an effective and highly selective inhibitor of the basal-like 2 (BL2) subtype of TNBC. Yuanhuacine can induce G2/M cell cycle arrest and has broad anti-tumor activity. Yuanhuacine is an orally active DNA damaging agent.
    Yuanhuacine
  • HY-161619
    RMC-113
    		Inhibitor 99.54%
    RMC-113 is a PIP4K2C and PIKfyve inhibitor with the Ki vaules of 46 nM and 370 nM. RMC-113 reverses SARS-CoV-2-induced impairment of autophagic flux. RMC-113 shows antiviral activity.
    RMC-113
  • HY-N0667S5
    L-Asparagine-d3 hydrate
    		99.64%
    L-Asparagine-d3 hydrate is the deuterium labeled L-Asparagine (HY-N0667). L-Asparagine is an essential amino acid for leukemic cells and a substrate for L-Asparaginase. L-Asparaginase is a potent anti-leukemic enzyme that promotes asparagine (Asn) and glutamine (Gln) depletion and inhibits protein biosynthesis in lymphoblasts. Removal of L-asparagine from plasma by L-Asparaginase results in inhibition of RNA and DNA synthesis and subsequent apoptosis. L-Asparaginase has cell-killing ability in vitro and in vivo, and selectively inhibits the growth of cancer cells with low asparagine synthetase (AASNS) expression. L-Asparagine can be used as a biomarker and sensor for the study of childhood acute lymphoblastic leukemia.
    L-Asparagine-d<sub>3</sub> hydrate
  • HY-N1584C
    Halofuginone lactate
    		Inhibitor 98.32%
    Halofuginone lactate, a Febrifugine derivative, is a competitive prolyl-tRNA synthetase inhibitor with a Ki of 18.3 nM. Halofuginone lactate is a specific inhibitor of type-I collagen synthesis and attenuates osteoarthritis (OA) by inhibition of TGF-β activity. Halofuginone lactate is also a potent pulmonary vasodilator by activating Kv channels and blocking voltage-gated, receptor-operated and store-operated Ca2+ channels. Halofuginone lactate has anti-malaria, anti-inflammatory, anti-cancer, anti-fibrosis effects.
    Halofuginone lactate
  • HY-E70094
    T4 UvsX Recombinase
    		99.9%
    T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates.
    T4 UvsX Recombinase
  • HY-136499
    DI-82
    		Inhibitor 98.0%
    DI-82 is a potent deoxycytidine kinase (dCK) inhibitor with an IC50app of 27.8 nM and Kiapp of 9.2 nM. DI-82 has antitumor activity.
    DI-82
  • HY-135282
    DHODH-IN-1
    		Inhibitor 98.65%
    DHODH-IN-1 (compound 18d) is a potent Dihydroorotate Dehydrogenase (DHODH) inhibitor with an IC50 of 25 nM. DHODH-IN-1 is an inhibitor of pyrimidine biosynthesis pathway.
    DHODH-IN-1
  • HY-N2384A
    Febrifugine dihydrochloride
    		Inhibitor 99.95%
    Febrifugine dihydrochloride is a quinazolinone alkaloid that inhibits P. falciparum and has antimalarial activity. Febrifugine dihydrochloride inhibits bladder cancer by inhibiting DNA synthesis, inducing apoptosis, and reducing steroids.
    Febrifugine dihydrochloride
  • HY-157417
    G-quadruplex ligand 1
    		98.62%
    G-quadruplex ligand 1, a G-quadruplex ligand, disrupts G-Quadruplex DNA structure and enhances gene expression.
    G-quadruplex ligand 1
  • HY-Y0958S
    Methoxyamine-d3 Hydrochloride
    		Inhibitor 99.54%
    Methoxyamine-d3 (O-Methylhydroxylamine-d3) hydrochloride is the deuterium labeled Methoxyamine hydrochloride. Methoxyamine hydrochloride is an orally active and potent base excision repair (BER) inhibitor.
    Methoxyamine-d<sub>3</sub> Hydrochloride
  • HY-W004883
    3-Amino-4,6-dimethylpyridine
    		Inhibitor 99.87%
    3-Amino-4,6-dimethylpyridine (5A-DMP) is a UHRF1 inhibitor that targets the Arg-binding cavity site within the UHRF1 TTD domain. 3-Amino-4,6-dimethylpyridine disrupts the interaction between UHRF1 and LIG1, interfering with DNA methylation, and can be utilized in cancer research.
    3-Amino-4,6-dimethylpyridine
  • HY-151224
    Thermospermine
    		Activator 99.97%
    Thermospermine, a structural isomer of Spermine, is synthesized by a thermospermine synthase designated ACAULIS5 (ACL5). Thermospermine suppresses auxin-inducible xylem differentiation in Arabidopsis thaliana.
    Thermospermine
  • HY-W028350
    NSC727447
    		Inhibitor
    NSC727447 is an inhibitor of ribonuclease H (Rnase H) of HIV-1 and HIV-2. NSC727447 has little activity against E. coli RNase H, but great selectivity over human Rnase H, with IC50s value of 2.0 μM, 2.5 μM, 100 μM, 10.6 μM, respectively.
    NSC727447
  • HY-145975A
    m7Gpppm6AmpG ammonium solution (100 mM)
    		98.26%
    m7Gpppm6AmpG ammonium solution (100 mM) is a trinucleotide mRNA 5’ cap analogs. m7Gpppm6AmpG ammonium can be used for RNA synthesis in vitro.
    m7Gpppm6AmpG ammonium solution (100 mM)
  • HY-W006102
    Bz-rA Phosphoramidite
    		99.03%
    Bz-rA Phosphoramidite (DMT-2'O-TBDMS-rA(bz) Phosphoramidite) a phosphoramidite containing 5' DMT and the nucleoside adenosine (A) with Benzoyl (Bz) base protection, for use in oligonucleotide synthesis.
    Bz-rA Phosphoramidite
  • HY-15620
    Methylproamine
    		Modulator 98.90%
    Methylproamine is a DNA-binding radioprotector, acts by repair of transient radiation-induced oxidative species on DNA. Methylproamine also protects against ionizing radiation by preventing DNA double-strand breaks.
    Methylproamine
  • HY-158714
    3'-ONH2-dCTP sodium solution (100 mM)
    		99.0%
    3'-ONH2-dCTP sodium solution (100 mM) is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3'-ONH2-dCTP sodium solution (100 mM)
  • HY-115736A
    Xanthosine-5'-Triphosphate trisodium solution (100 mM)
    		98.13%
    Xanthosine-5'-Triphosphate (5'-XTP) trisodium, a nucleotide, is produced by deamination of purine bases. Xanthosine-5'-Triphosphate trisodium is a substrate of inosine triphosphate pyrophosphatase (ITPase).
    Xanthosine-5'-Triphosphate trisodium solution (100 mM)
  • HY-19743
    Triazavirin
    		Inhibitor 99.89%
    Triazavirin is a nucleoside analogue of nucleic acid and an antiviral agent. Triazavirin works by inhibiting the synthesis of viral RNA and DNA and replication of genomic fragments. Triazavirin is also an effective protective agent on the transmission stage of influenza.
    Triazavirin
  • HY-Y1055S1
    Guanine-13C,15N2
    		98.6%
    Guanine-13C,15N2 is the 13C and 15N labeled Guanine (HY-Y1055). Guanine is one of the fundamental components of nucleic acids (DNA and RNA). Guanine is a purine derivative, consisting of a fused pyrimidine-imidazole ring system with conjugated double bonds. Guanine has the potential to serve as a large-capacity N pool. Guanine has cytotoxic, antinociceptive and neuroprotective effects.
    Guanine-<sup>13</sup>C,<sup>15</sup>N<sub>2</sub>
Cat. No. Product Name / Synonyms Application Reactivity
DNA/RNA Synthesis Isoform Comparison

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